Table I Experimental Variables at Different Levels Used for the Production of Gentamicin by Micromonouspora Echinospora Subs Pallida Using Ccd
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چکیده
The process parameters, temperature, pH and substrate concentration, were optimized for the production of gentamicin using Micromonouspora echinospora. Experiments were carried out according to central composite design in response surface method. The optimum conditions for the maximum production of gentamicin were found to be: temperature – 31.7C, pH – 6.8 and substrate concentration – 3%. At these optimized conditions the production of gentamicin was found to be – 1040 mg/L. The R value of 0.9465 indicates a good fitness of the model. Keywords—Gentamicin, Micromonouspora echinospora, response surface method, optimization, central composite design.
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